diff --git a/csharp/src/Drivers/Apache/Hive2/HiveServer2StandardConnection.cs b/csharp/src/Drivers/Apache/Hive2/HiveServer2StandardConnection.cs
index 4f89a39047..f15fd5741d 100644
--- a/csharp/src/Drivers/Apache/Hive2/HiveServer2StandardConnection.cs
+++ b/csharp/src/Drivers/Apache/Hive2/HiveServer2StandardConnection.cs
@@ -112,19 +112,15 @@ protected override TTransport CreateTransport()
             TTransport baseTransport;
             if (TlsOptions.IsTlsEnabled)
             {
-                X509Certificate2? trustedCert = !string.IsNullOrEmpty(TlsOptions.TrustedCertificatePath)
-                    ? new X509Certificate2(TlsOptions.TrustedCertificatePath!)
-                    : null;
-
                 RemoteCertificateValidationCallback certValidator = (sender, cert, chain, errors) => HiveServer2TlsImpl.ValidateCertificate(cert, errors, TlsOptions);
 
                 if (IPAddress.TryParse(hostName!, out var ipAddress))
                 {
-                    baseTransport = new TTlsSocketTransport(ipAddress, portValue, config: new(), 0, trustedCert, certValidator);
+                    baseTransport = new TTlsSocketTransport(ipAddress, portValue, config: new(), 0, null, certValidator);
                 }
                 else
                 {
-                    baseTransport = new TTlsSocketTransport(hostName!, portValue, config: new(), 0, trustedCert, certValidator);
+                    baseTransport = new TTlsSocketTransport(hostName!, portValue, config: new(), 0, null, certValidator);
                 }
             }
             else
diff --git a/csharp/src/Drivers/Apache/Hive2/HiveServer2TlsImpl.cs b/csharp/src/Drivers/Apache/Hive2/HiveServer2TlsImpl.cs
index 9a9311d7af..836f7d46dd 100644
--- a/csharp/src/Drivers/Apache/Hive2/HiveServer2TlsImpl.cs
+++ b/csharp/src/Drivers/Apache/Hive2/HiveServer2TlsImpl.cs
@@ -22,6 +22,7 @@
 using System.Net.Http;
 using System.Net.Security;
 using System.Security.Cryptography.X509Certificates;
+using System.Text.RegularExpressions;
 
 namespace Apache.Arrow.Adbc.Drivers.Apache.Hive2
 {
@@ -138,6 +139,30 @@ static internal TlsProperties GetStandardTlsOptions(IReadOnlyDictionary<string,
             return tlsProperties;
         }
 
+        public static List<X509Certificate2> LoadPemCertificates(string pemPath)
+        {
+            List<X509Certificate2> certs = new();
+            string pemContent = File.ReadAllText(pemPath);
+
+            MatchCollection matches = Regex.Matches(
+                pemContent,
+                "-----BEGIN CERTIFICATE-----(.*?)-----END CERTIFICATE-----",
+                RegexOptions.Singleline);
+
+            foreach (Match match in matches)
+            {
+                string base64 = match.Groups[1].Value
+                    .Replace("\r", "")
+                    .Replace("\n", "")
+                    .Trim();
+
+                byte[] rawData = Convert.FromBase64String(base64);
+                certs.Add(new X509Certificate2(rawData));
+            }
+
+            return certs;
+        }
+
         static internal bool ValidateCertificate(X509Certificate? cert, SslPolicyErrors policyErrors, TlsProperties tlsProperties)
         {
             if (policyErrors == SslPolicyErrors.None || tlsProperties.DisableServerCertificateValidation)
@@ -155,14 +180,39 @@ static internal bool ValidateCertificate(X509Certificate? cert, SslPolicyErrors
                 return !policyErrors.HasFlag(SslPolicyErrors.RemoteCertificateChainErrors) || (tlsProperties.AllowSelfSigned && IsSelfSigned(cert2));
             }
 
-            X509Certificate2 trustedRoot = new X509Certificate2(tlsProperties.TrustedCertificatePath);
             X509Chain customChain = new();
-            customChain.ChainPolicy.ExtraStore.Add(trustedRoot);
             // "tell the X509Chain class that I do trust this root certs and it should check just the certs in the chain and nothing else"
             customChain.ChainPolicy.VerificationFlags = X509VerificationFlags.AllowUnknownCertificateAuthority;
+            var collection = LoadPemCertificates(tlsProperties.TrustedCertificatePath!);
+
+            foreach (var trustedCert in collection)
+            {
+                customChain.ChainPolicy.ExtraStore.Add(trustedCert);
+            }
             customChain.ChainPolicy.RevocationMode = X509RevocationMode.Online;
 
             bool chainValid = customChain.Build(cert2);
+            if (chainValid)
+            {
+                bool trustedBy = false;
+                foreach (X509ChainElement element in customChain.ChainElements)
+                {
+                    foreach (X509Certificate2 ca in collection)
+                    {
+                        if (element.Certificate.Thumbprint == ca.Thumbprint)
+                        {
+                            trustedBy = true;
+                            break;
+                        }
+                    }
+                    if (trustedBy)
+                    {
+                        break;
+                    }
+                }
+                chainValid = chainValid && trustedBy;
+            }
+
             return chainValid || (tlsProperties.AllowSelfSigned && IsSelfSigned(cert2));
         }
     }