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b_hsqcf2et_1Hcoupled.cw
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;b_hsqcf2et_1Hcoupled.cw
;1H,13C BEST-HSQC with 1H coupling
;gradient-selected (echo/anti-echo)
;evolution times adjusted for zero 1H and 13C phase correction (AQMOD digital)
;with decoupling during acquisition
;
;modified Chris Waudby 15/10/16
;
;$CLASS=HighRes
;$DIM=2D
;$TYPE=
;$SUBTYPE=
;$COMMENT=
prosol relations=<triple>
#include <Avance.incl>
#include <Grad.incl>
#include <Delay.incl>
"d11=30m"
"d12=20u"
"d21=1s/(cnst2*4)"
"p4=2*p3"
"in0=inf1"
"d0=3u"
"DELTA=p17+d16-d0"
"DELTA1=d21-p17-d16-0.5*larger(p40,p4)-p39*cnst39"
"DELTA2=d21-p17-d16-0.5*larger(p40,p4)-p39*cnst39"
"DELTA3=d21-p17-d16-0.5*larger(p40,p4)-de-8u"
"acqt0=de"
"spoff23=bf1*(cnst19/1000000)-o1"
"spoff24=bf1*(cnst19/1000000)-o1"
1 ze
d11 pl12:f2
2 d1 do:f2
3 d12 pl2:f2
50u UNBLKGRAD
; purge equilibrium 13C
(p3 ph1):f2
4u
p16:gp5
d16
(p39:sp23 ph1):f1
p17:gp1
d16
DELTA1
;(center (p40:sp24 ph1):f1 (p3 ph11 p4 ph12 p3 ph11):f2 ) ; composite 13C inversion
(center (p40:sp24 ph1):f1 (p3*2 ph1):f2 )
DELTA1
p17:gp1
d16
(p39:sp23 ph2):f1
; zz purge
p16:gp3
200u
; t1 evolution
(p3 ph3):f2
d0
DELTA
(p4 ph5):f2
p17:gp6*EA
d16
(p3 ph4):f2
; zz purge
p16:gp4
200u
(p39:sp23 ph1):f1
p17:gp2
d16
DELTA2
(center (p40:sp24 ph1):f1 (p3*2 ph1):f2 )
DELTA3
p17:gp7
d16
4u BLKGRAD
4u pl12:f2
go=2 ph31 cpd2:f2
d1 do:f2 mc #0 to 2
F1EA(igrad EA, id0 & ip3*2 & ip31*2)
exit
ph1=0
ph2=1
ph3=0 2
ph4=0 0 2 2
ph5=0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 3
ph11=0
ph12=1
ph31=0 2 2 0 2 0 0 2
;pl2 : f2 channel - power level for pulse (default)
;pl12: f2 channel - power level for CPD/BB decoupling (low power)
;sp23: f1 channel - shaped pulse 120 degree
; (Pc9_4_120.1000 or Q5.1000)
;sp24: f1 channel - shaped pulse 180 degree (Rsnob.1000)
;p16: homospoil/gradient pulse [1 msec]
;p3: f2 channel - 90 degree high power pulse
;p39: f1 channel - 90 degree shaped pulse for excitation
; Pc9_4_90.1000 (90o) (3.0ms at 600.13 MHz)
;p40: f1 channel - 180 degree shaped pulse for refocussing
; Rsnob.1000 (1.0ms at 600.13 MHz)
;d0 : incremented delay (2D) = in0/2-p3*4/3.1415
;d1 : relaxation delay
;d11: delay for disk I/O [30 msec]
;d12: delay for power switching [20 usec]
;d16: delay for homospoil/gradient recovery
;d21 : 1/(2J)NH
;cnst2: = J(CH) [125 Hz for methyls]
;cnst19: H(N) chemical shift (offset, in ppm)
;cnst39: compensation of chemical shift evolution during p39
; Pc9_4_90.1000: 0.529
;inf1: 1/SW(C) = 2 * DW(C)
;in0: 1/ SW(C) = 2 * DW(C)
;nd0: 1
;NS: 4 * n
;DS: 16
;aq: <= 50 msec
;td1: number of experiments
;FnMODE: States-TPPI, TPPI, States or QSEC
;cpd2: decoupling according to sequence defined by cpdprg2: garp4
;pcpd2: f2 channel - 90 degree pulse for decoupling sequence
;for z-only gradients:
;gpz1: 11%
;gpz2: 7%
;gpz3: 9%
;gpz4: 17%
;gpz5: 13%
;gpz6: 80%
;gpz7: 27.1% (20.1% + gpz2)
;use gradient files:
;gpnam1: SINE.20
;gpnam2: SINE.20
;gpnam3: SMSQ10.100
;gpnam4: SMSQ10.100
;gpnam5: SMSQ10.100
;gpnam6: SINE.20
;gpnam7: SINE.20
;preprocessor-flags-start
;preprocessor-flags-end
;Processing
;PHC0(F1): 0
;PHC1(F1): 0
;FCOR(F1): 0.5
;$Id: sfhmqcf3gpph,v 1.1.2.8 2009/11/18 11:19:58 ber Exp $