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Platform: Linux (Ubuntu 22.04.1 LTS) UART: CH340-E Target: ATtiny1616 Behavior: flash completes first time successfully; subsequent attempts fail.
pymcuprog.serialupdi.physical - INFO - Opening port '/dev/ttyUSB0' at 115200 baud (timeout 1.0s)
pymcuprog.serialupdi.physical - ERROR - Unable to open serial port '/dev/ttyUSB0'
unplugging / pluggin-in the USB-UART accomplish some sort of reset and it will work again, once.
Note: The same test under Windows 10 x64 works every time and does not require the USB-UART to bet manually reset.