diff --git a/tests/opentelemetry-docker-tests/tests/boto/test_boto_functional.py b/tests/opentelemetry-docker-tests/tests/boto/test_boto_functional.py
new file mode 100644
index 0000000000..3e9e3d9c9a
--- /dev/null
+++ b/tests/opentelemetry-docker-tests/tests/boto/test_boto_functional.py
@@ -0,0 +1,55 @@
+# Copyright The OpenTelemetry Authors
+#
+# Licensed under the Apache License, Version 2.0 (the "License");
+# you may not use this file except in compliance with the License.
+# You may obtain a copy of the License at
+#
+#     http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing, software
+# distributed under the License is distributed on an "AS IS" BASIS,
+# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+# See the License for the specific language governing permissions and
+# limitations under the License.
+
+import unittest
+
+import boto3
+
+from opentelemetry.instrumentation.boto import BotoInstrumentor
+from opentelemetry.trace import NoOpTracerProvider, get_tracer_provider
+
+
+class TestBotoInstrumentationNoOpTracerProvider(unittest.TestCase):
+    def setUp(self):
+        # Set the NoOpTracerProvider
+        self.noop_tracer_provider = NoOpTracerProvider()
+        BotoInstrumentor().instrument(
+            tracer_provider=self.noop_tracer_provider
+        )
+
+    def tearDown(self):
+        BotoInstrumentor().uninstrument()
+
+    def test_boto_with_noop_tracer_provider(self):
+        # Create a boto3 client
+        client = boto3.client("s3")
+
+        # Perform a simple operation
+        try:
+            client.list_buckets()
+        except Exception:
+            pass  # Ignore any exceptions for this test
+
+        # Ensure no spans are created
+        tracer = get_tracer_provider().get_tracer("test")
+        if isinstance(self.noop_tracer_provider, NoOpTracerProvider):
+            # NoOpTracerProvider does not support span processing
+            self.assertTrue(True)
+        else:
+            spans = tracer.get_span_processor().spans
+            self.assertEqual(len(spans), 0)
+
+
+if __name__ == "__main__":
+    unittest.main()