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<div id="root">Please enable JavaScript to view this page. If you are viewing this page with javascript enabled, please contact
    wkwok16@uw.edu and send a screenshot of the developer console. Here is our project abstract:<br /><br />

    <h1>Chemically Induced Dimerization of Nanobodies for the Development of Versatile Biosensors</h1>

    One of the pressing challenges of modern science is the detection of small molecule targets. This has applications in a variety
    of fields, such as point-of-care diagnostics and metabolic engineering. Although most small molecules can be detected
    through Enzyme-Linked Immunosorbent Assay (ELISA), accessibility is limited by the time-consuming nature of the procedure.
    We hope to use a chemically induced dimerization system of nanobodies to detect small molecule targets and apply this
    system to the development of simple diagnostic assays, such as lateral flow assays or fluorescent microarrays, and innovations
    in metabolic engineering.<br /><br /> Nanobodies are antibody fragments from single domain antibodies found in camelids
    and sharks. They are the variable regions of antibodies that are responsible for specific binding to target molecules.
    Researchers in the Institute for Protein Design (IPD) at the University of Washington have created extensive libraries
    of phages displaying nanobodies for high throughput screening of binding targets, making them a versatile tool for molecule
    detection. We have partnered with Dr. Liangcai Gu from the IPD to investigate novel applications of a high-throughput
    nanobody library numbering ten billion unique types. In our project, we will identify specific nanobodies that bind to
    desired target molecules of high importance and demonstrate the application of nanobody technology to the development
    of a biosensor.<br /><br /> Traditionally, monoclonal antibodies have been used to detect specific molecules and used
    in products such as ELISA kits. However, one significant barrier to developing new monoclonal antibodies is that they
    require introduction of an antigen to an animal before large scale production can begin. We are bypassing this expensive
    and time-consuming step and the associated ethical concerns by screening through a pre-existing library of nanobodies
    to rapidly identify those that can bind desired target molecules with high specificity. <br /><br /> Our team will be
    screening our nanobody library against two chosen molecules to find binding nanobodies. Then, we will screen the library
    for secondary nanobodies that bind to the primary nanobody-antigen complex, thus forming a dimer. After identifying these
    nanobodies, these nanobodies can be fused to other proteins to form biosensors. Specifically, we intend to repurpose
    activating and DNA binding domain proteins used in yeast 2-hybrid systems to create a transcriptional biosensor.


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