Skip to content
This repository was archived by the owner on May 22, 2026. It is now read-only.

WIP: Added multi-allelic processing for amplicon mode#109

Open
PolinaBevad wants to merge 1 commit into
AstraZeneca-NGS:masterfrom
PolinaBevad:fix_part_of_issue_55_add_multi_allelic_amplicon
Open

WIP: Added multi-allelic processing for amplicon mode#109
PolinaBevad wants to merge 1 commit into
AstraZeneca-NGS:masterfrom
PolinaBevad:fix_part_of_issue_55_add_multi_allelic_amplicon

Conversation

@PolinaBevad
Copy link
Copy Markdown
Contributor

Have to be reviewed by Zhongwu Lai.

Description

This PR adds the same changes for amplicon multi-allelic cases as were added previously in Java version here: AstraZeneca-NGS/VarDictJava#155
For now, Ampbias flag will test all variants on all amplicon, included multi-allelic (so it have to go through all variants in amplicons and not the first one on the position as before). AMPFLAG determining fixed for this criteria.
Also, it will fix part of #55 issue (missed SNV because of multi-allelic skipping, but not Deletion problem which occurs in realignlgdel()),
PR branch was tested on dataset (1,6 Gb BAM file and Gene_Lung_primers.bed).

@PolinaBevad PolinaBevad changed the title Added multi-allelic processing for amplicon mode in Perl. Added multi-allelic processing for amplicon mode Apr 26, 2019
@PolinaBevad
Copy link
Copy Markdown
Contributor Author

A test case for amplicon run: https://drive.google.com/open?id=10pRdrqJwN8XisHA3QTHlyIQgAEmzXCXO
There are 2 variants at position 934144 but in the current version, only C->G variant will be printed.

@PolinaBevad PolinaBevad changed the title Added multi-allelic processing for amplicon mode WIP: Added multi-allelic processing for amplicon mode Jul 11, 2019
Sign up for free to subscribe to this conversation on GitHub. Already have an account? Sign in.

Labels

None yet

Projects

None yet

Development

Successfully merging this pull request may close these issues.

1 participant